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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+ii+1st+strand+cdna+synthesis+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11018 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer.
    Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210A, Kusatsu, Japan). qRT-PCR was performed using TB Green https://doi.org/10.3390/cells15090787 Fast qPCR Mix (Takara, RR420) on a CFX96 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Bile Canalicular Bitter Taste Receptors Inhibit β-Adrenergic Receptor-Induced Lipolysis in Steatotic Hepatocytes.
    Article Snippet: Total RNA was extracted from Hep3B2.1-7 cells, MPHs, and adipose tissue using the Trizol reagent (Invitrogen, 15596026), which was further purified using another columnbased RNA Extraction Kit (Takara, Shiga, Japan, 9767) to eliminate any residual genomic DNA. .. Approximately 3 μg of total RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210). .. Quantitative PCR reactions were set up using the primers listed in Supplementary Table S1 and iTaq Universal SYBR Green Supermix (Bio-rad, Hercules, CA, USA, 1725124) and performed on the CFX Connect Real-Time System.

    Article Title: Development of Two Multienzyme Isothermal Rapid Amplification Techniques for the Rapid Visual Detection of Micropterus salmoides rhabdovirus.
    Article Snippet: Largemouth bass (Micropterus salmoides) is an important freshwatercultured species in China.. M. salmoides rhabdovirus (MSRV) is a devastating pathogen that causes high mortality (exceeding 90% in acute outbreaks) in largemouth bass fry, posing a severe threat to the global largemouth bass aquaculture industry.. To address the urgent demand for rapid, onsite MSRV detection, two multienzyme isothermal rapid amplification (MIRA)derived methods—multienzyme isothermal rapid amplification with fluorescence detection (MIRAFLU) and multienzyme isothermal rapid amplification with lateral flow dipstick (MIRALFD)—were established and optimized targeting the conserved nucleoprotein (N) gene of MSRV.

    Article Title: Siglec-15 dysregulation and its therapeutic implications in new-onset type 1 diabetes
    Article Snippet: The stained sections were analyzed by a FLUOVIEW FV3000 laser scanning confocal microscope (Nikon Corporation) for high-resolution micro-imaging. .. Total RNA was extracted from MSCs, Control-MSCs, and Siglec15-MSCs cultured to 70-90% confluence using TRIzol ® Reagent (cat. no. 15596018; Thermo Fisher Scientific, Inc.) A total of 3 μ g of RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (cat. no. 6210A; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. The cDNA was further used to conduct qPCR analysis per the BeyoFast SYBR Green qPCR Mix (cat. no. D7260; Beyotime Biotechnology).

    cDNA Synthesis:

    Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer.
    Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210A, Kusatsu, Japan). qRT-PCR was performed using TB Green https://doi.org/10.3390/cells15090787 Fast qPCR Mix (Takara, RR420) on a CFX96 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Molecular epidemiology and genetic characteristics of influenza viruses in a local pediatric population of eastern China, 2024
    Article Snippet: Viral RNA was extracted from positive swabs using the MiniBEST Viral RNA/DNA Extraction Kit (TaKaRa) according to the manufacturer's instructions. .. Complementary DNA (cDNA) was synthesized with influenza A and B universal primers using the PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa). ..

    Article Title: A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa
    Article Snippet: Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) and then treated with RNase-free DNase I (Qiagen) to eliminate residual genomic DNA. .. First-strand cDNA was synthesized from 500 ng of total RNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa, Kusatsu, Japan) with oligo(dT) primers. ..

    Article Title: Involvement Of tRNA Thiolation In uORF-Mediated Translational Regulation During Xylogenesis In Arabidopsis thaliana.
    Article Snippet: .. First-strand cDNA synthesis was performed using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) according to the manufacturer’s instructions. .. The resulting cDNA was diluted 100-fold and used as template for real-time PCR with the KAPA SYBR Fast qPCR Kit (KAPA Biosystems, MA, USA) on a Thermal Cycler Dice Real Time System Lite (Takara).

    Article Title: Bile Canalicular Bitter Taste Receptors Inhibit β-Adrenergic Receptor-Induced Lipolysis in Steatotic Hepatocytes.
    Article Snippet: Total RNA was extracted from Hep3B2.1-7 cells, MPHs, and adipose tissue using the Trizol reagent (Invitrogen, 15596026), which was further purified using another columnbased RNA Extraction Kit (Takara, Shiga, Japan, 9767) to eliminate any residual genomic DNA. .. Approximately 3 μg of total RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210). .. Quantitative PCR reactions were set up using the primers listed in Supplementary Table S1 and iTaq Universal SYBR Green Supermix (Bio-rad, Hercules, CA, USA, 1725124) and performed on the CFX Connect Real-Time System.

    Article Title: Molding the Rice Methylome for Disease Resistance
    Article Snippet: Total RNA was extracted from 6d rice seedling leaves using the Maxwell 16 LEV Plant RNA kit (Promega) according to the manufacturer instructions. .. One μg of total RNA was then converted to cDNA using PrimeScript II 1st strand cDNA Synthesis Kit (Takara). ..

    Article Title: Development of Two Multienzyme Isothermal Rapid Amplification Techniques for the Rapid Visual Detection of Micropterus salmoides rhabdovirus.
    Article Snippet: Largemouth bass (Micropterus salmoides) is an important freshwatercultured species in China.. M. salmoides rhabdovirus (MSRV) is a devastating pathogen that causes high mortality (exceeding 90% in acute outbreaks) in largemouth bass fry, posing a severe threat to the global largemouth bass aquaculture industry.. To address the urgent demand for rapid, onsite MSRV detection, two multienzyme isothermal rapid amplification (MIRA)derived methods—multienzyme isothermal rapid amplification with fluorescence detection (MIRAFLU) and multienzyme isothermal rapid amplification with lateral flow dipstick (MIRALFD)—were established and optimized targeting the conserved nucleoprotein (N) gene of MSRV.

    Article Title: Siglec-15 dysregulation and its therapeutic implications in new-onset type 1 diabetes
    Article Snippet: The stained sections were analyzed by a FLUOVIEW FV3000 laser scanning confocal microscope (Nikon Corporation) for high-resolution micro-imaging. .. Total RNA was extracted from MSCs, Control-MSCs, and Siglec15-MSCs cultured to 70-90% confluence using TRIzol ® Reagent (cat. no. 15596018; Thermo Fisher Scientific, Inc.) A total of 3 μ g of RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (cat. no. 6210A; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. The cDNA was further used to conduct qPCR analysis per the BeyoFast SYBR Green qPCR Mix (cat. no. D7260; Beyotime Biotechnology).

    Quantitative RT-PCR:

    Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer.
    Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210A, Kusatsu, Japan). qRT-PCR was performed using TB Green https://doi.org/10.3390/cells15090787 Fast qPCR Mix (Takara, RR420) on a CFX96 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer.
    Article Snippet: .. One microgram of RNA was reverse-transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210A, Kusatsu, Japan). qRT-PCR was performed using TB Green https://doi.org/10.3390/cells15090787 Fast qPCR Mix (Takara, RR420) on a CFX96 real-time PCR system (Applied Biosystems, Foster City, CA, USA). ..

    Synthesized:

    Article Title: Molecular epidemiology and genetic characteristics of influenza viruses in a local pediatric population of eastern China, 2024
    Article Snippet: Viral RNA was extracted from positive swabs using the MiniBEST Viral RNA/DNA Extraction Kit (TaKaRa) according to the manufacturer's instructions. .. Complementary DNA (cDNA) was synthesized with influenza A and B universal primers using the PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa). ..

    Article Title: A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa
    Article Snippet: Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) and then treated with RNase-free DNase I (Qiagen) to eliminate residual genomic DNA. .. First-strand cDNA was synthesized from 500 ng of total RNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa, Kusatsu, Japan) with oligo(dT) primers. ..

    Control:

    Article Title: Siglec-15 dysregulation and its therapeutic implications in new-onset type 1 diabetes
    Article Snippet: The stained sections were analyzed by a FLUOVIEW FV3000 laser scanning confocal microscope (Nikon Corporation) for high-resolution micro-imaging. .. Total RNA was extracted from MSCs, Control-MSCs, and Siglec15-MSCs cultured to 70-90% confluence using TRIzol ® Reagent (cat. no. 15596018; Thermo Fisher Scientific, Inc.) A total of 3 μ g of RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (cat. no. 6210A; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. The cDNA was further used to conduct qPCR analysis per the BeyoFast SYBR Green qPCR Mix (cat. no. D7260; Beyotime Biotechnology).

    Cell Culture:

    Article Title: Siglec-15 dysregulation and its therapeutic implications in new-onset type 1 diabetes
    Article Snippet: The stained sections were analyzed by a FLUOVIEW FV3000 laser scanning confocal microscope (Nikon Corporation) for high-resolution micro-imaging. .. Total RNA was extracted from MSCs, Control-MSCs, and Siglec15-MSCs cultured to 70-90% confluence using TRIzol ® Reagent (cat. no. 15596018; Thermo Fisher Scientific, Inc.) A total of 3 μ g of RNA was used for reverse transcription using the PrimeScript II 1st Strand cDNA Synthesis Kit (cat. no. 6210A; Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. .. The cDNA was further used to conduct qPCR analysis per the BeyoFast SYBR Green qPCR Mix (cat. no. D7260; Beyotime Biotechnology).



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